The "sense" strand is used in transcription to RNA, so it is the one that makes sense to decode. The "antisense" is the complimentary strand, so it is just the opposite. The DNA enzyme (DNAzyme) 10–23 (Fig. 1a), also known as Dz10–23 or simply 10–23, is the best characterized example of a Mg 2+-dependent RNA-cleaving DNA enzyme created by in vitro selection Viruses are small obligate intracellular parasites, which by definition contain either a RNA or DNA genome surrounded by a protective, virus-coded protein coat. Viruses may be viewed as mobile genetic elements, most probably of cellular origin and characterized by a long co-evolution of virus and host. For propagation viruses depend on specialized host cells supplying the complex metabolic and 10.3390/ijms21093252. Aptamers or chemical antibodies are single-stranded DNA or RNA oligonucleotides that bind proteins and small molecules with high affinity and specificity by recognizing tertiary or quaternary structures as antibodies. Aptamers can be easily produced in vitro through a process known as systemic evolution of ligands by One of the most significant problems facing developmental biologists who do not work on an organism with well-developed genetics - and even for some who do - is how to inhibit the action of a gene of interest during development so as to learn about its normal biological function. A widely adopted approach is to use antisense technologies, and especially morpholino antisense oligonucleotides The purified linear DNA is now ready to use for in vitro transcription and can be stored at − 20°C for later use. B. Probe synthesis. Antisense probe synthesis is performed by in vitro transcription reaction using a DIG RNA labeling mix, an appropriate transcription buffer, and the appropriate RNA polymerase (an RNase inhibitor can also be Definition, Designing Technology, and Mechanism Antisense drugs are drugs that seek to block DNA transcription or RNA translation in order to moderate many disease processes. Antisense drugs consist of nucleotides linked together in short DNA or RNA sequences known as oligonucleotides. Oligonucleotides are designed knowing the target DNA/RNA to bind to specific DNA or RNA sequences or regions Antisense technology has been widely used as a potential therapeutic option to inhibit the specific gene expression. The antisense ODNs 3 play an indispensable role in the treatment of many diseases including cancer, influenza, and acquired immunodeficiency syndrome . The antisense ODNs, usually 18–21 nucleotides in length, specifically GHNj.